Inhibition of in vitro lymphocyte proliferation by brain glycoprotein (NSA3) and mesenchyme-associated antigen (MAA)
✍ Scribed by D. Gilbert; B. Delpech; A. Chevrier; C. Halavent
- Publisher
- Springer-Verlag
- Year
- 1981
- Tongue
- English
- Weight
- 467 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0340-7004
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✦ Synopsis
Two cross-reacting antigens, one (NSA3) associated with nervous system and the other (MAA) present in mesenchyme and different types of tumors, were shown to inhibit the mitogenic effect of PHA, Con-A, and PPD on normal peripheral human lymphocytes. They do not affect the response to allogeneic cells in the one-way mixed lymphocyte reaction. The two antigens also suppress in vitro response to PPD obtained with lymphocytes isolated from BCG-treated patients. The lack of effect on unstimulated lymphocytes and on MLR allows the exclusion of cytotoxic action by our antigen preparations. These data, taken in conjunction with previously obtained results concerning inhibition of E active rosette formation, give rise to the hypothesis of an antigen action directed against a T cell subpopulation. These results also suggest that MAA, in association with other tissue and tumor factors, could play a role in tumor-induced immunosuppression.
[14]. The role of tumor-associated antigens in tumor-induced immunosuppression has long been a subject of study. Alpha-fetoprotein has been shown to suppress certain T cell-dependent functions, such as allogeneic and mitogen-induced lymphocyte transformation [20,24,25]. An immunosuppressive activity of the alpha-2H isoferritin has also been demonstrated [3,4], which led us to investigate the possibility of such activity in our preparations of MAA and NSA3. The human peripheral blood lymphocyte (HPBL) responses to mitogens, antigen such as PPD, and allogeneic cells were then studied in the presence of MAA and NSA 3. Lymphocytes isolated from BCG-treated melanoma patients have been used as effector cells to induce a secondary response in vitro to PPD. We present here results that suggest the hypothesis that MAA and NSA3 exhibit suppressive activity on mitogen-or PPD-induced lymphocyte transformation.