## Abstract Results of hemacytometer cell counts and of tyrosinase measurements made by the Pomerantz method demonstrate that imidazole added to the medium of cultured B 16 mouse melanoma cells can stimulate tyrosinase specific activity and inhibit cell division. These effects are greater than with
✦ LIBER ✦
Inhibition of imidazole-induced tyrosinase activity by estradiol and estriol in cultured B 16/C3 melanoma cells
✍ Scribed by Ellis L. Kline; Terry J. Smith; Karen A. Carland; Barbara Blackmon
- Publisher
- John Wiley and Sons
- Year
- 1988
- Tongue
- English
- Weight
- 584 KB
- Volume
- 134
- Category
- Article
- ISSN
- 0021-9541
No coin nor oath required. For personal study only.
✦ Synopsis
Imidazole was supplied by Fisher Scientific (Pittsburgh, PA). All chemicals used were of the highest purity available.
Cell culture
B16/C3 mouse melanoma cell cultures were incubated at 37°C in 25-cm2 plastic flasks (Corning Medical, Med-
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## Abstract Incubation of cultured B‐16 melanoma cells with 1‐methyl‐3‐isobutyl xanthine (MIX) produced a sustained rise in intracellular adenosine 3′,5′‐cyclic monophosphate (cAMP) which preceded an increase in the specific activity of tyrosinase (EC 1.10.3.1). Cultures of two clones of melanoma c