Inhibition of human tumour cell proliferation by analogues of adenosine

✍ Scribed by Alison Colquhoun; Eric A. Newsholme

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 102 KB
• Volume: 15
• Category: Article
• ISSN: 0263-6484
• DOI: 10.1002/(sici)1099-0844(19970601)15:2<135::aid-cbf733>3.0.co;2-g

✦ Synopsis

The eects of adenosine and several structural analogues of adenosine upon thymidine incorporation into human tumour cells and rat cervical lymphocytes were investigated. The analogue NECA, which has equal speci®city for the A 1 and A 2 receptor, had the most inhibitory eect on lymphocyte proliferation while the A 1 agonists had limited eects, suggesting that these cells possess principally A 2 adenosine receptors. In the case of human tumour cells, however, the most inhibitory eect on proliferation was obtained with the A 1 -specific analogues. The general order of inhibitory eects of adenosine analogues on thymidine incorporation in human tumour cells was: S-ENBA b CPA R-PIA b S-PIA b NECAX These ®ndings suggest that in the cells presently studied the A 1 adenosine receptor predominates. Removal of exogenous adenosine by growth in the presence of adenosine deaminase inhibited thymidine incorporation. The eect of adenosine removal lends further support to the proposal that adenosine has some, as yet unidenti®ed, regulatory role in the control of human tumour cell proliferation.