Inhibition of human mitochondrial aldehyde dehydrogenase by the disulfiram metabolite S-methyl-N,N-diethylthiocarbamoyl sulfoxide: Structural characterization of the enzyme adduct by HPLC-tandem mass spectrometry

Identification of the protein-drug adduc

Identification of the protein-drug adduct formed between aldehyde dehydrogenase and S-methyl-N,N-diethylthiocarbamoyl sulfoxide by on-line proteolytic digestion high performance liquid chromatography electrospray ionization mass spectrometry

✍ Maryann L. Shen; Kenneth L. Johnson; Dennis C. Mays; James J. Lipsky; Stephen Na 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 155 KB 👁 2 views

Disulfiram has been used clinically as an aversion therapy treatment for recovering alcoholics. One of its metabolites, S-methyl-N, N-diethylthiocarbamoyl sulfoxide (MeDTC-SO), is currently believed by some to be the active metabolite in vivo. We demonstrate in this report that MeDTC-SO is a potent