Inhibition of human erythroid colony-forming units by interleukin-1 is mediated by gamma interferon

✍ Scribed by Robert T. Means Jr.; Emmanuel N. Dessypris; Sanford B. Krantz

• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 647 KB
• Volume: 150
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041500109

✦ Synopsis

IL-l inhibits erythropoiesis in vivo and in vitro. This inhibition was studied by comparing the effect of recombinant human IL-l (rhlL-1) on highly purified CFU-erythroid (E) generated from peripheral blood burst-forming units-erythroid (BFU-E) (mean purity 44.4%) with its effect on unpurified marrow CFU-E (mean purity 0.36%). Colony formation by marrow CFU-E was significantly inhibited by rhlL-1, while colony formation by highly purified CFU-E was not inhibited. However, purified CFU-E colonies were inhibited by rhlL-1 in the presence of autologous T-lymphocytes, and also by cell-free conditioned medium prepared from T-lymphocytes stimulated by rhlL-1. This inhibitory effect was ablated by neutralizing antibodies to yinterferon (IFN), but not by antibodies to human IL-1, tumor necrosis factor, or PIFN. Colony formation by highly purified CFU-E was also inhibited by recombinant human ylFN (rhylFN). IL-1 and ylFN play significant roles in the pathogenesis of the anemia of chronic disease. These studies indicate that rhlL-1 inhibits CFU-E colony formation by an indirect mechanism involving T-lymphocytes and requiring ylFN and that ylFN itself is most probably the direct mediator of this effect.