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Inhibition of hepatitis A virus replication in vitro by antiviral compounds

✍ Scribed by J. M. Crance; E. Biziagos; J. Passagot; H. van Cuyck-Gandré; Dr. R. Deloince

Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
645 KB
Volume
31
Category
Article
ISSN
0146-6615

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✦ Synopsis

Abstract

Forty antiviral compounds were screened for inhibitory effect on hepatitis A virus (HAV) antigen expression in the human hepatoma cell line PLC/PRF/5. Ribavirin, amantadine, glycyrrhizin, and pyrazofurin were selected in this screening test and were studied further. The selectivity indices of these four compounds, calculated as the ratio of 50% cytotoxic dose (determined by the trypan blue exclusion and by inhibition of [^3^H] leucine incorporation) to the 50% effective dose (determined by the viral antigen expression), were 4.6 and 3.0 with ribavirin, 5.3 and 5.9 with amantadine, 15.2 and 16.9 with glycyrrhizin, and 45.4 and 74.6 with pyrazofurin. All four compounds resulted in concentration‐dependent reductions of HAV antigen expression and HAV infectivity. Ribavirin, amantadine, pyrazofurin, and glycyrrhizin emerged, from the present study, as promising candidates for chemotherapy of acute hepatitis A.

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