Inhibition of gap-junctional intercellular communication and enhanced binding of fibronectin-coated latex beads by stimulation of DNA synthesis in quiescent 3T3-L1 cells
✍ Scribed by Yoshiki Shiba; Yasuto Sasaki; Yoshinobu Kanno
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 1020 KB
- Volume
- 145
- Category
- Article
- ISSN
- 0021-9541
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✦ Synopsis
To clarify the modulation of intercellular communication via gap junctions, associated with the growth induction of quiescent 3T3-Ll cells, we investigated the gap-junctional intercellular communication in growth-stimulated cells that were able to bind fibronectin-coated beads. When quiescent 3T3-Ll cells were incubated with fibronectin-coated beads for the first 2 h after the addition of calf serum, 24.0% of the cells bound and phagocytosed beads. Among the cells with bound beads, the percentage of the cells labeled concurrently with bromodeoxyuridine was 63.7% when examined 13 h after the addition of calf serum.
Transient reduction of dye-coupling, measured with Lucifer Yellow CH, was observed only in the cells with bound beads 2 h after addition of calf serum, but it was not observed in the cells without bound beads. When the quiescent cells were incubated with fibronectin-coated beads for 2 h from 4-6 h after the addition of calf serum, the percentage of cells with bound beads increased to 53.1 %, but the decrease in dye-coupling among the cells with bound beads was slight. These results suggest that the induction of cell growth causes a transient reduction in gap-junctional intercellular communication in 3T3-Ll cells with bound fibronectin-coated beads.