Inhibition of cinnamyl-alcohol-dehydrogenase activity and lignin synthesis in poplar (Populus x euramericanaDode) tissues by two organic compounds
✍ Scribed by C. Grand; F. Sarni; A. M. Boudet
- Publisher
- Springer-Verlag
- Year
- 1985
- Tongue
- English
- Weight
- 518 KB
- Volume
- 163
- Category
- Article
- ISSN
- 0032-0935
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✦ Synopsis
Two organic compounds, N-(O-hydroxyphenyl)-and N-(O-aminophenyl)sulfinamoyltertiobutyl acetate (OHPAS and NHzPAS, respectively) have been designed for inhibiting cinnamylalcohol dehydrogenase (EC 1.1.1.2), a zinc metalloenzyme involved specifically in lignification. This paper describes their effects in vitro on the activity of the enzyme isolated from poplar and in vivo on the lignification of poplar tissues. The enzyme inhibition was time-and dose-dependent and pseudoirreversible indicating that these compounds could act as suicide inhibitors. Neither OHPAS nor NH2PAS exhibited affinity towards other plant zinc metalloenzymes or phenolic enzymes tested. Only NHzPAS exerted an effect on cinnamoyl: CoA reductase, another specific enzyme of lignification. In addition, these inhibitors, at the concentration of 80 gM, reduced the fluxes of lignin synthesis in poplar stems by 45%. These results show that OHPAS and NHzPAS could become useful tools for reducing lignification in plants.