Inhibition of cell proliferation and glucose uptake in human laryngeal carcinoma cells by antisense oligonucleotides against glucose transporter-1

✍ Scribed by Shui-Hong Zhou; Jun Fan; Xiao-Ming Chen; Ke-Jia Cheng; Shen-Qing Wang

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 464 KB
• Volume: 31
• Category: Article
• ISSN: 1043-3074
• DOI: 10.1002/hed.21137

✦ Synopsis

Abstract

Background.

Malignant cells show increased glucose uptake in vitro and in vivo, which is thought to be mediated by glucose transporters. In this study, we investigated the effect of plasmid‐derived antisense RNA against the Glut‐l gene on proliferation and glucose uptake in laryngeal carcinoma Hep‐2 cells.

Methods.

The expression plasmids pcDNA3.1(+)‐Glut‐1 and pcDNA3.1(+)‐anti Glut‐1 were constructed. The MTT method was used to assess cell growth inhibition. The expression of Glut‐1 mRNA and protein was detected by reverse transcriptase‐polymerase chain reaction and Western blotting, respectively.

Results.

After transfection, Glut‐1 AS clearly inhibited glucose uptake and cell growth in Hep‐2 cells, and we observed a decrease in the expression of Glut‐1 mRNA and protein in Hep‐2 cells.

Conclusions.

Glut‐1 AS decreases glucose uptake and inhibits the proliferation of Hep‐2 cells. © 2009 Wiley Periodicals, Inc. Head Neck, 2009