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Inhibition of cell-free messenger ribonucleic acid translation by 7-methylguanosine 5'-Triphosphate: effect of messenger ribonucleic acid concentration

โœ Scribed by Chu, Lee-Yun; Rhoads, Robert E.


Book ID
127286064
Publisher
American Chemical Society
Year
1980
Tongue
English
Weight
1005 KB
Volume
19
Category
Article
ISSN
0006-2960

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Determination of 7-methylguanosine-conta
โœ Hans-Joachim Breter; Lawrence T. Malek; Gary M. Hellmann; Robert E. Rhoads ๐Ÿ“‚ Article ๐Ÿ“… 1979 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 757 KB

A method is described for the determination of 5'-terminal methylated (cap) structures in unlabeled mRNA based on oxidation with NaIO.,, reduction with NaB["H],. cleavage with P, nuclease. and separation on a strong anion-exchange resin by high-performance liquid chromatography (hplc). Model compoun