Inhibition of CAT enzyme activity inArabidopsis thaliana

✍ Scribed by Markus Röver; Anja Matzk; Barbara Baker; Joachim Schiemann; Reinhard Hehl

• Book ID: 104620617
• Publisher: Springer
• Year: 1996
• Tongue: English
• Weight: 802 KB
• Volume: 45
• Category: Article
• ISSN: 0167-6857
• DOI: 10.1007/bf00043425

✦ Synopsis

Previously it was shown that transient chloramphenicol acetyltransferase (CAT) marker gene expression in Arabidopsis thaliana and Nicotiana tabacum resulted in significant differences in the accumulation of the CAT reaction products in radioactive CAT assays. Compared to Nicotiana tabacum, conversion of chloramphenicol to the acetylated products in Arabidopsis thaliana extracts was rather low. Here we report that the low CAT enzyme activity can be attributed in part to a heat sensitive CAT inhibitory effect in extracts ofArabidopsis thaliana. CAT enzyme acitvity in transgenic tobacco is inhibited by extracts from Arabidopsis. This inhibitory effect diminishes when Arabidopsis extracts were heat incubated. CAT activity in transgenic Arabidopsis lines was very low and was only detected in heat incubated extracts. Alternatively, enzyme-linked immunosorbent assays (ELISAs) can be used to detect the CAT protein in transgenic Arabidopsis.