Inhibition of carbohydrate oxidation during the first minute of reperfusion after brief ischemia: NMR detection of hyperpolarized 13CO2 and H13CO
✍ Scribed by Matthew E. Merritt; Crystal Harrison; Charles Storey; A. Dean Sherry; Craig R. Malloy
- Book ID
- 102534878
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 392 KB
- Volume
- 60
- Category
- Article
- ISSN
- 0740-3194
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✦ Synopsis
Abstract
Isolated rat hearts were studied by ^31^P NMR and ^13^C NMR. Hyperpolarized [1‐^13^C]pyruvate was supplied to control normoxic hearts and production of [1‐^13^C]lactate, [1‐^13^C]alanine, ^13^CO~2~ and H^13^CO was monitored with 1‐s temporal resolution. Hearts were also subjected to 10 min of global ischemia followed by reperfusion. Developed pressure, heart rate, oxygen consumption, [ATP], [phosphocreatine], and pH recovered within 3 min after the ischemic period. During the first 90 s of reperfusion, [1‐^13^C]alanine and [1‐^13^C]lactate appeared rapidly, demonstrating metabolism of pyruvate through two enzymes largely confined to the cytosol, alanine aminotransferase, and lactate dehydrogenase. ^13^CO~2~ and H^13^CO were not detected. Late after ischemia and reperfusion, the products of pyruvate dehydrogenase, ^13^CO~2~ and H^13^CO were easily detected. Using this multinuclear NMR approach, we established that during the first 90 s of reperfusion PDH flux is essentially zero and recovers within 20 min in reversibly‐injured myocardium. Magn Reson Med 60:1029–1036, 2008. © 2008 Wiley‐Liss, Inc.