Inhibition of bud initiation by ethylN-phenylcarbamate results in meiotic development in the yeastSaccharomyces cerevisiae
✍ Scribed by Kyoji Yamada; Michio Ito
- Book ID
- 104761214
- Publisher
- Springer
- Year
- 1983
- Tongue
- English
- Weight
- 721 KB
- Volume
- 134
- Category
- Article
- ISSN
- 0302-8933
No coin nor oath required. For personal study only.
✦ Synopsis
When diploid cells ofSaccharomyces cerevisiae were incubated in acetate growth media containing 2.5 mM ethyl N-phenylcarbamate (EPC), bud initiation was inhibited preferentially, and eventually overgrown, unbudded cells accumulated. During subsequent incubation, meiosis and ascospore formation occurred at high frequencies. The behavior of EPC-treated cells was essentially the same as that of cells transferred to a starvation sporulation medium. EPC thus has a pronounced effect on the mitotic growth of yeast cells, which leads to meiotic development. Our observations indicate that EPC has a decisive function in the initiation of meiosis in rich growth media.