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Inhibition of ascorbate oxidation by urate

✍ Scribed by Kwok-Wai Lam; Donald Fong; Andrew Lee; Keng Ming; D. Liu

Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
465 KB
Volume
22
Category
Article
ISSN
0162-0134

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A method for the detection of ascorbate peroxidase activity in native electrophoretic gels is described. The assay is based on the ability of ascorbate peroxidase to prevent the ascorbate-dependent reduction of nitroblue tetrazolium in the presence of \(\mathrm{H}_{2} \mathrm{O}_{2}\). The method wa