Inhibition of an HIV-1 Tat-derived peptide binding to TAR RNA by aminoglycoside antibiotics

Use of a Polyethylene Glycol–Peptide Con

Use of a Polyethylene Glycol–Peptide Conjugate in a Competition Gel Shift Assay for Screening Potential Antagonists of HIV-1 Tat Protein Binding to TAR RNA

✍ Jihong Wang; Shaei-Yun Huang; Indrani Choudhury; Michael J. Leibowitz; Stanley S 📂 Article 📅 1995 🏛 Elsevier Science 🌐 English ⚖ 139 KB

Interference of binding of Tat protein to TAR RNA in HIV-1-infected cells may be a useful therapeutic strategy for AIDS. An electrophoretic assay to screen potential low-molecular-weight (< 2 kDa) Tat antagonists has been established. A radiolabeled TAR RNA fragment (delta TAR) is retarded in mobili