Inhibition of all-trans retinoic acid-induced granulocytic differentiation of WEHI-3B D+ cells by forced expression of SCL (TAL1) and GATA-1
✍ Scribed by Kimiko Ishiguro; Anna M. Rice; Kevin P. Rice; Alan C. Sartorelli
- Publisher
- Elsevier Science
- Year
- 2009
- Tongue
- English
- Weight
- 696 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0145-2126
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✦ Synopsis
All-trans retinoic acid (ATRA) induces granulocytic maturation of WEHI-3B D+ leukemia cells and LiCl enhances this maturation, while WEHI-3B D- cells are non-responsive to ATRA. Transfection of SCL, expressed in D- but absent in D+ cells, into D+ cells, caused resistance to ATRA, while transfection of GATA-1 into D+ cells produced resistance to the combination of ATRA and LiCl. SCL expression in D+ cells did not induce the expression of c-Kit, a putative target gene for SCL. LiCl, known to inhibit some kinases by displacing Mg2+, did not affect tyrosine kinase activity of the cytoplasmic domain of c-Kit.