Functional assay for human CD4+CD25+ Tre
✍
Laura Tsaknaridis; Leslie Spencer; Nicole Culbertson; Kevin Hicks; Dorian LaToch
📂
Article
📅
2003
🏛
John Wiley and Sons
🌐
English
⚖ 266 KB
## Abstract CD4^+^CD25^+^ regulatory T cells (Treg cells) prevent T cell‐mediated autoimmune diseases in rodents. To develop a functional Treg assay for human blood cells, we used FACS‐ or bead‐sorted CD4^+^CD25^+^ T cells from healthy donors to inhibit anti‐CD3/CD28 activation of CD4^+^CD25^−^ ind