The inhibition by cysteine and glutathione of 4-amino antipyrine oxidation by horseradish peroxidase was compared. The kinetics of the peroxidase inhibition by glutathione was investigated and compared to l-cysteine inhibition. IC 50 for l-cysteine and glutathione was determined and used for the sub
β¦ LIBER β¦
Inhibition Mechanism of TbIII on Horseradish Peroxidase Activity
β Scribed by Shaofen Guo; Qing Zhou; Tianhong Lu; Xiaolan Ding; Xiaohua Huang
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 557 KB
- Volume
- 5
- Category
- Article
- ISSN
- 1612-1872
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## Abstract Phthalic anhydride (PA) modification stabilizes horseradish peroxidase (HRP) by reversal of the positive charge on two of HRP's six lysine residues. Native and PAβHRP had halfβinactivation temperatures of 51 and 65Β°C and halfβlives at 65Β°C of 4 and 17 min, respectively. PAβHRP was more
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