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Inhibition Assay for the Binding of Biotinylated von Willebrand Factor to Platelet-Bound Microtiter Wells in the Presence of Ristocetin or Botrocetin

✍ Scribed by S. Miura; S. Nishida; K. Makita; Y. Sakurai; T. Shimoyama; M. Sugimoto; A. Yoshioka; K. Ishii; M. Kito; T. Kobayashi; Y. Fujimura

Publisher: Elsevier Science
Year: 1996
Tongue: English
Weight: 139 KB
Volume: 236
Category: Article
ISSN: 0003-2697
DOI: 10.1006/abio.1996.0159

No coin nor oath required. For personal study only.

✦ Synopsis

kDa dispase-digested vWF fragment (residues Leu 480 / tein Ib; ELISA, enzyme-linked immunosorbent assay; SIPA, shear-Val 481 -Gly 718 of vWF subunit) and the recombinant induced platelet aggregation; BSA, bovine serum albumin; HBS, Hepes-buffered saline; PBS, phosphate-buffered saline. vWF fragment (MalE-rvWF 508 -704 ; residues Tyr 508 -215