Inhibition and stimulation of respiration-linked Mg2+efflux in rat heart mitochondria
✍ Scribed by Karl E. O. Åkerman
- Publisher
- Springer US
- Year
- 1981
- Tongue
- English
- Weight
- 350 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0145-479X
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## Abstract Liver mitochondria from normal and alloxan diabetic rats, isolated in 0.25 M sucrose, were assayed with an oxygen electrode for ADP/O and Ca^+2^/O ratios, respiratory ratio, and respiratory control index. Mitochondria were incubated with two substrates, succinate and β‐hydroxybutyrate;
Alterations in cytosolic metabolites and [ Mg2+], were monitored using 3'P magnetic resonance spectroscopy during inotropic stimulation of isolated rat heart [ 10 nMisoproterenol, 0.6 p M isobutyl-1-methylxanthine (IBMX), 5 pM ouabain]. All drugs significantly elevated contractile function (rate-pre