✦ LIBER ✦

Inheritance and structure of foreign DNA in progenies of transgenic tobacco obtained by direct gene transfer

✍ Scribed by H. Morota; H. Uchimiya

Publisher: Springer
Year: 1988
Tongue: English
Weight: 515 KB
Volume: 76
Category: Article
ISSN: 0040-5752
DOI: 10.1007/bf00257840

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✦ Synopsis

One of the transformed tobacco plants obtained by direct DNA transformation possessed two marker genes, a chimeric aminoglycoside phosphotransferase and nopaline synthase genes. Selfed progenies of this plant (T3-d) showed stable inheritance of these two genes. The minimum size of foreign DNA integrated into tobacco genome was estimated to be 5.4 kbp. A deleted nopaline synthase gene co-existed with an intact gene. The linkage analysis indicated that two transformants, T1-b and T3-c, possessed foreign DNA inserted in different chromosomes or in different sites of the same chromosome that recombine freely.

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