Inheritance and alteration of transforming DNA during an induced parasexual cycle in the imperfect fungusCladosporium fulvum

✍ Scribed by José Arnau; Richard P. Oliver

• Book ID: 104717273
• Publisher: Springer-Verlag
• Year: 1993
• Tongue: English
• Weight: 563 KB
• Volume: 23
• Category: Article
• ISSN: 0172-8083
• DOI: 10.1007/bf00312643

✦ Synopsis

Protoplasts of a pAN7-1-transformed isolate of Cladosporium fulvum race 4, harbouring a tandem duplication of the vector, and an untransformed race 5 isolate were fused, without selection for the presence of vector sequences. Fusion products were allowed to haploidize spontaneously. The inheritance of pAN7-1 sequences and the expression of the hph gene was studied in 85 progeny. A very high proportion (80%) of the progeny contained vector sequences and 70% of the progeny were resistant to hygromycin. Inactivation of the hph gene occurred in eight progeny. Rearrangement of vector sequences can account for the inactivation: there is no evidence for any RIP-like mechanism. A number of novel bands were observed.