Infrequent alterations of the p16INK4A gene in liver cancer

✍ Scribed by Ryuichi Kita; Naoshi Nishida; Yoshihiro Fukuda; Hidemasa Azechi; Yoko Matsuoka; Toshiki Komeda; Takehiro Sando; Kazuwa Nakao; Kanji Ishizaki

• Publisher: John Wiley and Sons
• Year: 1996
• Tongue: French
• Weight: 499 KB
• Volume: 67
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/(sici)1097-0215(19960717)67:2<176::aid-ijc4>3.0.co;2-q

✦ Synopsis

We examined the genomic status of the p161NK4A (inhibitor of cyclin-dependent kinase 4 A) and cyclin-dependent kinase 4 (CDK4) genes in 62 human hepatocellular carcinomas (HCCs), 5 cholangiocellular carcinomas and 6 cell lines derived from human liver canceo. Although no samples showed the homozygous deletion of the t161NK4A gene, we detected intragenic mutations of the p16" 4A gene in 3 HCCs and one HCC cell line, which led to an amino-acid substitution or a frameshift. In 2 HCC samples with mis-sense mutations of the ~1 6 " ~~~ gene, loss of heterozygosity on 9822 was also detected, suggesting that the loss of function of p I 6 was induced during hepatocarcinogenesis. On the other hand, amplification or rearrangement of the CDK4 gene was not detected in any samples examined in this study. These results indicated that the mutations or deletions of the p161NK4A gene are not frequent, but may play a role in a sub-set of human HCC.