Infrared study of phospholipid hydration. Main phase transition of saturated phosphatidylcholine/water multilamellar samples

✍ Scribed by André Mellier; Abdallah Diaf

• Book ID: 103040173
• Publisher: Elsevier Science
• Year: 1988
• Tongue: English
• Weight: 388 KB
• Volume: 46
• Category: Article
• ISSN: 0009-3084
• DOI: 10.1016/0009-3084(88)90113-2

✦ Synopsis

Infrared spectra of phosphatidylcholine (DMPC, DPPC, DSPC)/water multilamellar samples were recorded as a function of both water concentration and temperature. From wavenumber shifts, molecular interactions at the polar head/water interface are pointed out, particularly between the phosphate groups and the water molecules. Temperature profiles of the 6 CH~ (1465 cm -~) and 6 OH (1645 cm -t) absorption bands provide the main transition temperatures of both the hydrocarbon chains of the phospholipid and the water layer. Experimental evidence of a re-ordering of the water layer before the well-known melting of the phospholipid chains are shown, whatever the water concentration and the chain length may be.