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Infrared-Assisted On-Plate Proteolysis for MALDI-TOF-MS Peptide Mapping

✍ Scribed by Wang, Sheng; Bao, Huimin; Zhang, Luyan; Yang, Pengyuan; Chen, Gang


Book ID
127329090
Publisher
American Chemical Society
Year
2008
Tongue
English
Weight
493 KB
Volume
80
Category
Article
ISSN
0003-2700

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Alternating current (AC) has been employed to enhance the efficiency of chymotryptic proteolysis for peptide mapping. It was allowed to flow through the mixture solution of proteins and chymotrypsin via a pair of platinum wire electrodes. Bovine serum albumin (BSA) and cytochrome c (Cyt-c) were dige