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Informatics for mass spectrometry-based RNA analysis

✍ Scribed by Hiroshi Nakayama; Nobuhiro Takahashi; Toshiaki Isobe

Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
802 KB
Volume
30
Category
Article
ISSN
0277-7037

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Mass spectrometry (MS) allows the sensitive and direct characterization of biological macromolecules and therefore has the potential to complement the more conventional genetic and biochemical methods used for RNA characterization. Although MS has been used much less frequently for RNA research than it has been for protein research, recent technical improvements in both instrumentation and software make MS a powerful tool for RNA analysis because it can now be used to sequence, quantify, and chemically analyze RNAs. Mass spectrometry is particularly well suited for the characterization of RNAs associated with ribonucleoprotein complexes. This review focuses on the software and databases that can be used for MS‐based RNA studies. Software for the processing of raw mass spectra, the identification and characterization of RNAs by mass mapping, de novo sequencing, and tandem MS‐based database searching are available. Β© 2010 Wiley Periodicals, Inc., Mass Spec Rev 30:1000–1012, 2011

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