𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Influenza virus H5N1 hemagglutinin (HA) T-cell epitope conjugates: design, synthesis and immunogenicity

✍ Scribed by Theodore Skarlas; Stella Zevgiti; Karoline Droebner; Eugenia Panou-Pomonis; Oliver Planz; Maria Sakarellos-Daitsiotis

Publisher
John Wiley and Sons
Year
2010
Tongue
English
Weight
246 KB
Volume
17
Category
Article
ISSN
1075-2617

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The influenza virus, major surface glycoprotein hemagglutinin (HA) is one of the principal targets for the development of protective immunity. Aiming at contributing to the development of a vaccine that remains the first choice for prophylactic intervention, a reconstituted model of HA, mimicking its antigenic properties was designed, synthesized and tested in mice for the induction of protective immunity. Four helper T lymphocyte [HTL (T~1~, T~3~, T~7~ and T~8~)] and four cytotoxic lymphocyte [CTL (T~2~, T~4~, T~5~ and T~6~)] epitopes were coupled in two copies each to an artificial carrier, SOC~4~, which was formed by the repeating tripeptide Lys‐Aib‐Gly. The helical conformation of the SOC~4~‐conjugates preserves the initial topology of the attached epitopes, which is critical for their immunogenic properties. Survival of immunized animals, ranged from 30 to 50%, points out the induction of protective immunity by using the SOC~4~‐conjugates. Copyright © 2010 European Peptide Society and John Wiley & Sons, Ltd.

📜 SIMILAR VOLUMES

Sialic acid and sialyl-lactose glyco-con
Sialic acid and sialyl-lactose glyco-conjugates: design, synthesis and binding assays to lectins and swine influenza H1N1 virus
✍ Stella Zevgiti; Juliana Gonzalez Zabala; Ayub Darji; Ursula Dietrich; Eugenia Pa 📂 Article 📅 2011 🏛 John Wiley and Sons 🌐 English ⚖ 242 KB

The terminal parts of the influenza hemagglutinin (HA) receptors __α__2,6‐ and __α__2,3‐sialyllactoses were conjugated to an artificial carrier, named sequential oligopeptide carrier (SOC~4~), to formulate human and avian receptor mimics, respectively. SOC~4~, formed by the tripeptide unit Lys‐Aib‐G

Comparing the antibody responses against
Comparing the antibody responses against recombinant hemagglutinin proteins of avian influenza A (H5N1) virus expressed in insect cells and bacteria
✍ Shuo Shen; Geetha Mahadevappa; Hsueh-Ling Janice Oh; Boon Yu Wee; Yook-Wah Choi; 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 402 KB

## Abstract The hemagglutinin (HA) of influenza A virus plays an essential role in mediating the entry of the virus into host cells. Here, recombinant full‐length HA5 protein from a H5N1 isolate (A/chicken/hatay/2004(H5N1)) was expressed and purified from the baculovirus‐insect cell system. As expe