Influence of the explantation milieu on intranuclear [Na], [K] and [Mg] of Chironomus thummi salivary gland cells
✍ Scribed by Heinrich Kroeger; Walter Trösch
- Publisher
- John Wiley and Sons
- Year
- 1974
- Tongue
- English
- Weight
- 490 KB
- Volume
- 83
- Category
- Article
- ISSN
- 0021-9541
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✦ Synopsis
Abstract
Intranuclear Na, K and Mg concentrations were determined in cells of salivary glands incubated for 1^h^ in selected NaCl/KCl/MgCl~2~ media. By variation of the external milieu beyond “physiological” limits the intranuclear electrolytes can be shifted between ca 100 and 280 mM [K]^i^, between ca 8 and 100 mM [Na]^i^ and between ca 5 and 75 mM [Mg]^i^. No significant competition or interactions of the 3 ionic species are apparent. The relationships [K]^e^ : [K]^i^ and [Na]^e^ : [Na]^i^ can best be described by a positive and linear, that between [Mg]^e^ : [Mg]^i^ by a negative and exponential function. Regression parameters are given which permit a computation of intranuclear [Na], [K] and [Mg] as induced by NaCl/KCl/MgCl~2~ in any binary or triple combination that is tolerated by the explanted gland without visible damage.