Influence of the Amino Acid Residue Downstream of (Asp)4Lys on Enterokinase Cleavage of a Fusion Protein: Volume 269, Number 1 (1999), pages 10–16

Influence of the Amino Acid Residue Down

Influence of the Amino Acid Residue Downstream of (Asp)4Lys on Enterokinase Cleavage of a Fusion Protein

✍ Tanya Hosfield; Quinn Lu 📂 Article 📅 1999 🏛 Elsevier Science 🌐 English ⚖ 125 KB

We have studied the cleavage efficiency of the protease enterokinase (EK) using the novel vector pESP4. pESP4 is a yeast expression vector equipped with ligation-independent cloning sites, a GST purification tag, and a FLAG epitope tag. EK is used to cleave the FLAG and GST tags leaving the protein