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Influence of synthetic polymers on neutrophil migration in three-dimensional collagen gels

✍ Scribed by Tan, Jian ;Saltzman, W. Mark

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
772 KB
Volume
46
Category
Article
ISSN
0021-9304

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✦ Synopsis

In vitro studies of cell migration within threedimensional polymeric materials are essential for understanding cell behavior and for developing new biomedical materials. Human neutrophil motility was examined in hydrated collagen gels containing various synthetic polymers. Physical mixtures of collagen and certain water-soluble polymers formed stable gels that were good substrates for cell migration. Addition of either polyethylene glycol (PEG) or the pluronic™ copolymer F68 did not change the morphological or mechanical properties of collagen gels, as determined by SEM and oscillatory rheometry; however, addition of either polymer significantly inhibited cell motility in both a modified 96-well chemotaxis chamber assay and a direct visual assay. Although the mechanism for this observed polymer inhibition of neutrophil migration is not yet clear, these results suggest that PEG and F68, two widely used biomedical polymers that are considered to be relatively "inert," may cause significant inhibition of cell motility.

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