INFLUENCE OF SUCCESSIVE AND COMBINED ULTRAVIOLET A AND B IRRADIATIONS ON MATRIX METALLOELASTASES PRODUCED BY HUMAN DERMAL FIBROBLASTS IN CULTURE
✍ Scribed by C. KUT; W. HORNEBECK; N. GROULT; G. REDZINIACK; G. GODEAU; B. PELLAT
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 172 KB
- Volume
- 21
- Category
- Article
- ISSN
- 1065-6995
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✦ Synopsis
To study the cumulative influence of UV irradiations on skin matrix alterations, human skin fibroblasts were irradiated successively three-fold, at 24 h intervals, with UVA (3 5J/cm 2 ), UVB (3 8mJ/cm 2 ), UVA plus UVB (3 5J/cm 2 and 3 8mJ/cm 2 ) and the levels of 92 kDa gelatinase (pro-MMP 9 ), 72 kDa gelatinase (pro-MMP 2 ) and plasma-membrane elastase type protease were determined, following subsequent 24-h culture in 10% serum-containing medium. UV irradiations had only minor influence (1.4-fold increase for UVB) on secreted levels of pro-MMP 2 and decreased the amount of plasma membrane elastase produced by cells. It did however, for UVA and UVB alone, induce a significant increase of 66 kDa activated MMP 2 production: 2.5-and 1.7-fold respectively. Such enhancement was not observed when combined irradiations were administered. UV exposure possessed a much higher influence on pro-MMP 9 secretion by dermal fibroblast enhancing enzyme levels by 2.5-, 6.5-and 5-fold for UVA, UVB and UVA+UVB, respectively.