Influence of stereoisomeric glucose, galactose and mannose residues on fragmentation at their glycosidic linkages in post-source decay fragment analyses for oligosaccharides using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

✍ Scribed by Tohru Yamagaki; Hiroshi Nakanishi

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 123 KB
• Volume: 12
• Category: Article
• ISSN: 0951-4198
• DOI: 10.1002/(sici)1097-0231(19980831)12:16<1069::aid-rcm291>3.0.co;2-j

✦ Synopsis

The isomeric sugar branched b-cyclodextrin (CD) derivatives (Glc-bCD, Gal-bCD, and Man-bCD) were analyzed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. In the MALDI post-source decay (PSD) fragmentation of Glc-bCD, Gal-bCD and Man-bCD, the fragment ions of [MÀGlc] , [MÀGal] and [MÀMan] were produced by the one-site cleavage of the a1-6 glycosidic linkage at the branch (Y-type fragmentation). The abundances of [MÀGlc] and [MÀGal] ions were much higher than that of [MÀMan] . These results indicated that Glc-bCD and Gal-bCD were distinguishable from Man-bCD, and that Y-type fragmentations of the branched glycosidic linkage at the glycosyl donors of a-D-Glc and a-D-Gal were more predominant than that at the glycosyl donor of a-D-Man by MALDI-PSD fragment analysis. It was concluded that the abundances of PSD fragment ions depended on the stereochemistry of the glycosyl donors in the cleavage of the glycosidic linkage of the oligosaccharides in MALDI-TOF mass spectra.