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Influence of RNA secondary structure on HEV gene amplification using reverse-transcription and nested polymerase chain reaction

โœ Scribed by Shaojing Wei; Shing Shun Tony To


Book ID
117769633
Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
339 KB
Volume
27
Category
Article
ISSN
1386-6532

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## Abstract A procedure is described for the detection of hepatitis C virus (HCV) RNA in blood by means of the polymerase chain reaction (PCR) in which the reverse transcription step and two rounds of amplification are carried out in a single tube. This results in fewer manipulations, reduced risk