The blood kinetics and tissue distribution of a conjugate of daunomycin and a monoclonal antibody (791T/36) have been examined in mice, including nude mice with human tumour xenografts reactive with the antibody. For this the antibody moiety of the conjugate was labelled with 125I and the drug moiet
Influence of recombinant tumour necrosis factor a on blood flow and antibody localisation in human tumour xenografts in nude mice
โ Scribed by M. V. Pimm; S. J. Gribben; T. M. Morris
- Book ID
- 112574307
- Publisher
- Springer-Verlag
- Year
- 1991
- Tongue
- English
- Weight
- 634 KB
- Volume
- 117
- Category
- Article
- ISSN
- 1432-1335
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The blood kinetics and tumour localization of a conjugate of methotrexate (MTX) and MAb 791T136 were examined i n nude mice with human tumour xenografts. The antibod moiety of the conjugate was detected by labelling with l2J and the drug moiety was assayed using a radioimmunoassay for methotrexate.
To demonstrate the precise distribution and binding of in vivo injected monoclonal antibodies on histological tumour sections, we have biotinylated our primary antibody AUA I. Biotinylated antibody was injected into nude mice bearing simultaneous subcutaneous and intraperitoneal xenografts of the hu