Influence of photoperiod and medium formulation on peanut somatic embryogenesis

✍ Scribed by Charleen M. Baker; J. Austin Burns; Hazel Y. Wetzstein

• Publisher: Springer
• Year: 1994
• Tongue: English
• Weight: 493 KB
• Volume: 13-13
• Category: Article
• ISSN: 0721-7714
• DOI: 10.1007/bf00239884

✦ Synopsis

Somatic embryos were produced from peanut (Arachis hypogaea L.) immature zygotic cotyledons. Comparisons were made of the level of α-naphthaleneacetic acid during induction, nitrogen formulation of the medium, and photoperiod. Over 70% embryogenesis was obtained regardless of NAA level used. Percent embryogenesis and number of embryos were markedly lower in explants induced on NAA compared to 2,4-D. Embryo production was not greatly affected by either the use of Murashige & Skoog versus Finer & Nagasawa salts or light versus dark culture conditions. However, embryo morphology was noticeably affected by photoperiod. Embryos produced under a 16 h photoperiod were tough, woody and difficult to separate for subsequent germination and conversion. Those produced under a 0-h photoperiod were succulent and pliable.