Influence of phosphate on the formation of the indole alkaloids and phenolic compounds in cell suspension cultures ofCatharanthus roseusI. Comparison of enzyme activities and product accumulation
✍ Scribed by K-H. Knobloch; J. Berlin
- Publisher
- Springer
- Year
- 1983
- Tongue
- English
- Weight
- 425 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0167-6857
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✦ Synopsis
In cell suspension cultures of Catharanthus roseus a rapid accumulation of secondary compounds (tryptamine, indole alkaloids, phenolics) was observed after transfer of the cells into special 'induction'-media devoid of phosphate and other essential growth factors [11,14]. The increase of product levels was suppressed in the presence of phosphate which was almost completely taken up from the medium and accumulated by the cells within 48 h after inoculation. The activities of tryptophan decarboxylase (TDC), the first enzyme in indole alkaloid biosynthesis, and of phenylalanine ammonia-lyase (PAL), the key enzyme of phenytpropanoid biosynthesis, were influenced differently by phosphate. Whereas the accumulation of phenolics and PAL activity were similarly inhibited by low concentration of phosphate, the mediuminduced enhanced activity of TDC was not affected although the product pools were considerably reduced. Some consequences for the regulation of secondary metabolism will be discussed.