Influence of O2-carrying plasma hemoprotein “albumin-heme” on complement system and platelet activation in vitro and physiological responses to exchange transfusion
✍ Scribed by Teruyuki Komatsu; Yubin Huang; Shinobu Wakamoto; Hideki Abe; Mitsuhiro Fujihara; Hiroshi Azuma; Hisami Ikeda; Hisashi Yamamoto; Hirohisa Horinouchi; Koichi Kobayashi; Eishun Tsuchida
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 305 KB
- Volume
- 81A
- Category
- Article
- ISSN
- 1549-3296
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✦ Synopsis
Abstract
Recombinant human serum albumin (HSA) including the synthetic iron(II)‐porphyrin (FeP), albumin‐heme (HSA‐FeP), is a unique O~2~‐carrying plasma hemoprotein as a red blood cell substitute. We have investigated the possible influence of HSA‐FeP on the complement system and platelet activation in vitro. The amounts of the serum complement titer CH~50~ and terminal complement complex SC5b‐9 of human blood serum, incubated with HSA‐FeP (10, 20, and 40 vol %), were almost the same as those of the corresponding samples with HSA. The effect of HSA‐FeP on the platelet reactivity has been demonstrated by conformational changes in the membrane glycoprotein IIb/IIIa and surface expression of an α‐granule membrane protein P‐selectin. Platelet activation in response to the ADP‐stimulation was not influenced by the presence of HSA‐FeP. It can be concluded that the albumin‐heme solution does not facilitate the immunological reaction and platelet activation. Moreover, a 20% exchange transfusion with HSA‐FeP into anesthetized rats has been performed to evaluate the circulation and blood parameters for 6 h. Time course changes in all parameters showed features identical to the control group (without infusion) and HSA group. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2007