Influence of meristem-tip size and location on morphological development inDioscorea cayenensis-D. rotundatacomplex ‘Grosse Caille’ and one genotype ofD. praehensilis

✍ Scribed by Bernard Malaurie; Jean-Claude Thouvenel; Okito Pungu

• Publisher: Springer
• Year: 1995
• Tongue: English
• Weight: 262 KB
• Volume: 42
• Category: Article
• ISSN: 0167-6857
• DOI: 10.1007/bf00034240

✦ Synopsis

In vitro morphological development of meristem-tips, excised from microshoots, is reported for one clone 'Grosse Caille' ofD. cayenensis-D, rotundata complex and for one clone ofD. praehensilis. The effects of size and location of meristem-tip on viability and morphological development after 3, 8 and 11 months in vitro culture were studied. The larger size of meristem-tip gave the best rooting and plantlet formation while the smaller size gave the best bud production. Axillary buds were more reactive than apical meristem-tips in callusing and rooting when small-sized meristems of the 'G-rosse Caille' clone were used; the use of larger meristem-tips did not induce more differences between apical and axillary buds. The production of plantlets was successful, after 11 months of culture, with a score of 82 and 39 % survivors, for 'Grosse Caille' clone and D. praehensilis genotype, respectively.