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Influence of Ligands on the Coenzyme Dissociation Constants in Octopine Dehydrogenase

✍ Scribed by Antonio BAICI; Pier Luigi LUISI; Anna OLOMUCKI; Marie-Odile DOUBLET; Jozef KLINCAK

Book ID
115114223
Publisher
John Wiley and Sons
Year
1974
Tongue
English
Weight
667 KB
Volume
46
Category
Article
ISSN
1432-1327

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✍ Peter Brodelius; Klaus Mosbach πŸ“‚ Article πŸ“… 1976 πŸ› Elsevier Science 🌐 English βš– 511 KB

Various alcohol and lactate dehydrogenases were adsorbed to an affinity column of immobilized W-(6-aminohexyl)-AMP and subsequently eluted with gradients of coenzymes or coenzyme fragments. A linear relation was observed between the eluting concentration of nucleotide and the reported dissociation c