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Influence of inorganic phosphate on the activity determination of isoenzymes of alkaline phosphatase in various buffer systems

โœ Scribed by Klaus Jung; Monika Pergande

Book ID
115822930
Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
355 KB
Volume
102
Category
Article
ISSN
0009-8981

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Inorganic phosphate determination in the
Inorganic phosphate determination in the presence of a labile organic phosphate: Assay for carbamyl phosphate phosphatase activity
โœ Michael J. Black; Mary Ellen Jones ๐Ÿ“‚ Article ๐Ÿ“… 1983 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 455 KB

An assay was developed for carbamyl phosphate phosphatase (CAP phosphatase) activity. This enzyme activity is difficult to assay because of the chemical instability of carbamyl phosphate (CAP). We chose to measure CAP phosphatase activity by monitoring release of inorganic phosphate (Pi). This entai