Influence of inorganic phosphate and organic buffers on cephalosporin production byStreptomyces clavuligerus
✍ Scribed by Yair Aharonowitz; Arnold L. Demain
- Publisher
- Springer
- Year
- 1977
- Tongue
- English
- Weight
- 380 KB
- Volume
- 115
- Category
- Article
- ISSN
- 0302-8933
No coin nor oath required. For personal study only.
✦ Synopsis
A high concentration of potassium phosphate (75-100 mM) stabilized pH and supported extensive growth of Streptomyces clavuligerus in a chemically defined medium; such a concentration also inhibited cephalosporin production. Although Tris buffer was found to have detrimental effects on growth and antibiotic production, 3-(N-morpholino)propane sulfonate (MOPS) or 2-(N-morpholino)ethane sulfonate (MES) buffer provided a nontoxic buffering system. In the presence of MOPS buffer, cephalosporin production was optimal at 25 mM phosphate, whereas higher concentrations of phosphate progressively inhibited antibiotic production up to 85 ~o without modifying the pH pattern. MOPS buffer can be used to conduct fermentations at a relatively constant pH value in shake flasks.