Influence of hydroxyapatite particle size on bone cell activities: Anin vitro study

✍ Scribed by Sun, Jui-Sheng ;Liu, Hwa-Chang ;Hong-Shong Chang, Walter ;Li, Jimmy ;Lin, Feng-Huei ;Tai, Han-Cheng

• Publisher: John Wiley and Sons
• Year: 1998
• Tongue: English
• Weight: 77 KB
• Volume: 39
• Category: Article
• ISSN: 0021-9304
• DOI: 10.1002/(sici)1097-4636(19980305)39:3<390::aid-jbm7>3.0.co;2-e

✦ Synopsis

Over the past decade, a large number of biomaterials have been proposed as artificial bone fillers for repairing bone defects. The material most widely used in clinical medicine is hydroxyapatite. The aim of our investigation was to study the effect of hydroxyapatite size mechanism on osteoblasts.

The osteoblasts were cultured in vitro with 0.1% (1 mg/ mL) of various sized hydroxyapatite particles (0.5-3.0, 37-63, 177-250, and 420-841 m) for 1 h, 3 h, 1 day, 3 days, and 7 days. The results showed that adding hydroxyapatite particles to osteoblast culturs can significantly affect osteoblast cell count. Osteoblast populations decreased significantly. Osteoblast mean surface areas also changed significantly.

Transforming growth factor-␤1 (TGF-␤1) concentrations in culture medium decreased significantly with the addition of hydroxyapatite particles. Prostaglandin E 2 (PGE 2 ) concentrations in medium increased significantly. The changes in TGF-␤1 and PGE 2 concentration were more significant and persisted longer in smaller-particle groups. The inhibitory effects of hydroxyapatite particles on osteoblast cell cultures were mediated by the increased synthesis of PGE 2 . Caution should be exercised before using a hydroxyapatite product which could easily break down into fine particles.