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Influence of human papillomavirus type 16 gene expression on in vitro differentiation of the human teratocarcinoma cell line 2102Ep

✍ Scribed by Hubert Stöppler; Tatjana Kirchhoff; Jürgen Kartenbeck; Lutz Gissmann; Angel Alonso

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 916 KB
Volume: 16
Category: Article
ISSN: 0899-1987
DOI: 10.1002/(sici)1098-2744(199606)16:2<109::aid-mc7>3.0.co;2-d

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✦ Synopsis

Human papillomaviruses (HWs) are known to infect human keratinocytes and cause alterations in epithelial differentiation. We showed in this study that expression of the HPV-16 genome was able to interfere with the in vitro differentiation of a human simple-epithelial cell type, the 2102Ep teratocarcinoma cell line. Stable HPV-16 genome-expressing 2102Ep cell lines were generated, and subsequent alterations in differentiation were analyzed in comparison with parental 2102Ep cells. We found that in 2102Ep cells phorbol ester-induced differentiation led to changes in the expression of SSEA antigens, whereas in HPV-transfected cell lines only minor changes were observed.

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