Influence of GSTT1 genotype on sister chromatid exchange induction by styrene-7,8-oxide in cultured human lymphocytes
✍ Scribed by Tiina Ollikainen; Ari Hirvonen; Hannu Norppa
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 120 KB
- Volume
- 31
- Category
- Article
- ISSN
- 0893-6692
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✦ Synopsis
The genetic polymorphisms of glutathione S-trans-(50 mM) and 1.4 (150 mM) times greater among ferases (GSTs), which are involved in the metabolic the GSTT1 null individuals (4.83 at 50 mM, 18.98 inactivation of various toxicants, have been sug-at 150 mM) compared with the GSTT1 positive indigested to be an important source of variation in viduals (2.78 at 50 mM, 13.74 at 150 mM), the individual response to genotoxic carcinogens. We differences being statistically significant (P Å 0.006 have previously shown that donor GSTM1 geno-and P Å 0.022, respectively). These findings show type does not influence the induction of sister chro-that the lack of the GSTT1 gene increases the genomatid exchanges (SCEs) in cultured human lympho-toxic effects of SO in human whole-blood lymphocytes by styrene-7,8-oxide (SO), a metabolite of sty-cyte cultures, suggesting that GSTT1 is involved in rene. Here, we expanded the study to GSTT1 the detoxification of SO in humans. Although glutapolymorphism. SCEs were analyzed from 72-hr thione conjugation is considered a minor metabolic whole-blood lymphocyte cultures of five GSTT1 posi-pathway for SO in vivo, the high GSTT1 activity in tive (at least one undeleted allele) and five GSTT1 erythrocytes may be important locally and might null (gene homozygously deleted) donors, all affect the level of genotoxic damage observed in GSTM1 positive, after a 48-hr treatment with 50 peripheral lymphocytes of styrene-exposed rein-mM and 150 mM SO. SO clearly increased SCEs forced plastics workers. The GSTT1 polymorphism in cultures of all donors. The mean number of SCEs/ could also influence the urinary excretion of SOcell induced by SO (individual mean SCEs from specific mercapturic acids. Environ. Mol. Mutagen. acetone-treated control cultures subtracted) was 1.