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Influence of growth temperature on the acid phosphatase activity in the yeast Yarrowia lipolytica

✍ Scribed by E. Vasileva-Tonkova; D.M. Balasheva; D. Galabova

Book ID
115539954
Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
416 KB
Volume
145
Category
Article
ISSN
0378-1097

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An acid phosphatase from derepressed cells of the yeast form of Yarrowia lipolyrica was purified 176-fold by ammonium sulfate precipitation, chromatography on DEAE-Sephacel and gel filtration in Biogel A 5-M. The enzyme is a glycoprotein with a sugar content of 60%. The MICHAELIS constant of the enz