Influence of glucose on adenine nucleotide levels and energy charge in Acetobacter aceti

✍ Scribed by B�chi, Brigitte ;Ettlinger, L.

• Publisher: Springer-Verlag
• Year: 1973
• Weight: 541 KB
• Volume: 93
• Category: Article
• ISSN: 0003-9276
• DOI: 10.1007/bf00424946

✦ Synopsis

The influence of glucose on adenine nucleotide levels and energy charge in a growing culture of Acetobacter aceti and a glucose sensitive mutant, whose growth on ethanol is inhibited by glucose, was determined.

1. Both strains showed an abrupt fall of the intracellular ATP after substrate exhaustion.

2. Glucose alone could not maintain the energy charge in either strain at the high values observed during growth on ethanol.

3. Glucose lowered the total adenine nucleotide content in the mutant in the absence of ethanol.

4. During the inhibition of growth by glucose in the glucose sensitive mutant, three phases could be distinguished: a) A first period with a transient decline in the total adenine nuclcotide level and no change in the energy charge.

b) A recovery of the total adenine nucleotide level to its previous value. This second phase lasted as long as there was ethanol in the medium. c) A third period after ethanol exhaustion characterized by a lower total adenine nucleotide level in the presence of glucose and a lower energy charge.