Influence of fetal bovine serum on cytotoxic and genotoxic effects of lectins in MCF-7 cells

✍ Scribed by Glaucia Veríssimo Faheina-Martins; Alethéia Lacerda da Silveira; Márcio Viana Ramos; Luís Fernando Marques-Santos; Demetrius Antonio Machado Araujo

• Publisher: John Wiley and Sons
• Year: 2011
• Tongue: English
• Weight: 311 KB
• Volume: 25
• Category: Article
• ISSN: 1095-6670
• DOI: 10.1002/jbt.20388

✦ Synopsis

Abstract

Canavalia ensiformis (ConA), Canavalia brasiliensis (Conbr), and Cratylia floribunda (CFL) lectins have exhibited glucose‐mannose binding specificity. We investigated the effect of fetal bovine serum (FBS) concentrations (1, 5, 10, and 20%) on the cytotoxic effect of these lectins against breast tumor cell line MCF‐7. Cell viability was examined using the MTT reduction assay. When cells were grown in a medium supplemented with a higher serum concentration (10 or 20%), all lectins were much less toxic. When we used 1% FBS, it was possible to achieve a concentration‐dependent activity by all examined lectins, with an IC~50~ of 3.5, 25, and 60 µg/mL for ConA, Conbr, and CFL, respectively. All lectins incubated with 1% FBS induced apoptosis and DNA damage in MCF‐7 cells. We conclude that ConA, Conbr, and CFL lectins' cytotoxic and genotoxic effects were observed only at low concentrations of serum. © 2011 Wiley Periodicals, Inc. J Biochem Mol Toxicol 25:290–296, 2011; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.20388