Influence of feeding strategies of mixed microbial cultures on the chemical composition and microstructure of copolyesters P(3HB-co-3HV) analyzed by NMR and statistical analysis
✍ Scribed by G. Ivanova; L. S. Serafim; P. C. Lemos; A. M. Ramos; M. A. M. Reis; E. J. Cabrita
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 181 KB
- Volume
- 47
- Category
- Article
- ISSN
- 0749-1581
- DOI
- 10.1002/mrc.2423
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✦ Synopsis
Abstract
NMR spectroscopy was applied for quantitative and qualitative characterization of the chemical composition and microstructure of a series of poly(3‐hydroxybutyrate‐co‐3‐hydoxyvalerate) copolymers, P(3HB‐co‐3HV), synthesized by mixed microbial cultures at several different feeding strategies. The monomer sequence distribution of the bacterially synthesized P(3HB‐co‐3HV) was defined by analysis of their high‐resolution 1D ^13^C NMR and 2D ^1^H/^13^C HSQC and ^1^H/^13^C HMBC NMR spectra. The results were verified by employment of statistical methods and suggest a block copolymer microstructure of the P(3HB‐co‐3HV) copolymers studied. Definitive distinction between block copolymers or a mixture of random copolymers could not be achieved. NMR spectral analysis indicates that the chemical composition and microstructure of the copolymers can be tuned by choosing a correct feeding strategy. Copyright © 2009 John Wiley & Sons, Ltd.