TWENTY-NINE FIGURES ## INTRODUCTIOK Extensive surveys of free amino acids of animal tissues by two-dimensional paper chromatography have shown that in a given species at a particular stage of development' each normal tissue, including every type of blood cell, has a distribution of free or loosel
Influence of extraction procedure on size of free amino acid pool of heart muscle
β Scribed by Keith L. Manchester
- Publisher
- Elsevier Science
- Year
- 1980
- Tongue
- English
- Weight
- 287 KB
- Volume
- 102
- Category
- Article
- ISSN
- 0003-2697
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β¦ Synopsis
More free leucine, isoleucine, methionine, and phenylalanine and possibly valine and tyrosine was extracted from frozen pulverized heart muscle by heating in water at 100Β°C followed by deproteinization with trichloroacetic acid at 0Β°C than when the powder was extracted with trichloroacetic acid at 0Β°C. The question of which procedure estimates the true pool of free amino acids in the tissue is discussed.
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Five sire types, namely Danish Duroc (DU), Dutch Large White (LW D ), English Large White (LW E ), Belgian Landrace Γ Landrace (BL Γ LR) and Belgian Landrace (BL), were mated with LR Γ LW crossbred sows. Dipeptidylpeptidase and aminopeptidase activities and free amino acid and natural dipeptide cont