Influence of ePTFE polymer implant permeability on the rate and density of corneal extracellular matrix synthesis
✍ Scribed by Legeais, Jean-Marc ;Drubaix, Isabelle ;Briat, Benoit ;Savoldelli, Mich�le ;M�nasche, Maurice ;Robert, Ladislas ;Renard, Gilles ;Pouliquen, Yves
- Publisher
- John Wiley and Sons
- Year
- 1997
- Tongue
- English
- Weight
- 282 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0021-9304
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✦ Synopsis
Microporous polymers have great potential for the production of corneal keratoprosthetic devices. Keratocytes invade the pores of expanded polytetrafluoroethylene implants (ePTFE) and collagen synthesis occurs. This ePTFE becomes translucent after its implantation in the stroma of rabbit cornea. The rate and density of cell growth within this polymer depends on the implant thickness, pore size, and its placement in the cornea. We have investigated the influence of the polymer permeability on the collagen and protein contents of ePTFE implants. Rabbit corneal stroma were implanted with ePTFE disks (6 mm in diameter) by intralamellar keratoplasty. The implanted polymers were removed from the stroma after 3 or 6 months. The collagen and pro-tein contents were determined after pepsin solubilization. The collagen content of the high-permeability implant was 3.7-fold greater than that of the low-permeability implant 3 months after implantation and 2.4-fold greater after 6 months. The total protein content of the high-permeability implant was 2.5-fold greater than that of the lowpermeability implant at 3 months and was the same after 6 months. The collagen-to-protein ratio was 68% in the highpermeability implants, and thus similar to that of normal corneal stroma. Thus, high polymer permeability increased both the rate and density of the corneal extracellular matrix ingrowth.